Gyn Biomarkers

Gynecologic biomarkers testing click to access further explanations in the original CAP template

Biomarkers assessments :

- For ER/PR, POSITIVE is >= 1%
- For HER2, no consensus yet, use MODIFIED CA breast criteria.
- For PD-L1, CPS = Total positive cells / (Positive cells + Negative tumor cells). CPS >=1 is POSITIVE.

- Estrogen recepter (ER) IHC :
- Estrogen recepter (ER) IHC :	- Average Intensity :
- Progesterone recepter (PR) IHC :
- Progesterone recepter (PR) IHC :	- Average Intensity :
- HER2/neu oncoprotein IHC : Low-power, observed within a homogeneous and contiguous population of invasive tumor cells.
	- Score :
+ HER2/neu oncoprotein DISH : HER2 DISH calculator NEGATIVE: FISH HER2/CEP17 ratio less than 2.0 and Average HER2 copy number < 6/nucleus POSITIVE: FISH HER2/CEP17 ratio greater than or equal to 2.0 OR FISH HER2/CEP17 ratio less than 2.0 with average HER2 copy number >= 6/nucleus
	- Number of tumor cells counted :
	- Average HER2 signals (black) per nucleus :
	- Average CEP17 signals (red) per nucleus :
	- HER2/CEP17 ratio :
	- Other ISH abnormalities: Aneusomy Heterogeneous signals
- p53 IHC:
- MMR IHC:
	- MLH1 (mutL homolog 1):
	- MSH2 (mutS homolog 2):
	- MSH6 (mutS homolog 6):
	- PMS2 (PMS1 homolog 2):
+ MSI status: *Note that hereditary colon cancer clinic is using slightly different markers for MSI. Six mononucleotide repeat (BAT25, BAT26, SLC7A8 (NR-21), TMPPB5 (NR-22), ZNF-2 (NR-24) and BAT40), and three dinucleotide repeats (D2S123, D5S346, D17S250).
	BAT-25:
	BAT-26:
	NR-21:
	NR-24 :
	NR-27 :
	D2S123:
	D5S346:
	D17S250:
+ PD-L1 assessment : PD-L1 calculator
	- Antibody (anti-CD274) :
	- Positive tumor cells (TPS, %) :
	- Combined Positive Score (CPS) :

+ Ancillary studies include BRCA1 and BRCA2 germline and somatic mutation testings and POLE hot spot mutations

DOWNLOAD THE EXCEL FILE for HER2 DISH

DOWNLOAD THE EXCEL FILE for PD-L1 IHC test

- Additional notes :