List of PCR tests available in the laboratory.

CAP recommends using neoplastic cellularity as the number of neoplastic cells or nuclei compared to the overall or toal number of cells or nuclei.

DNA is extracted from micro-dissected tumor-rich tissue area on FFPE section. PCR is performed on exons with hot spot mutations and the PCR products are analysized by Sanger sequencing using capillary electrophoresis. DNA integrity is checked across PCR products of sizes 135, 226, 315 and 435 bp. Tumor content >20% is ideal for molecular analysis. Micro-dissection allows enrichment of more than 80% of tumor cells, exceeding the general accepted sensitivity of 15% for manual sequence.

Automated real-time PCR, digital PCR and droplet digital PCR are also available for tests requiring high sensitivity.

References databases:
1. Atlas of Genetics and Cytogenetics in Oncology and Haematology
2. Catalogue of Somatic Mutations in Cancer COSMIC, databases in the Wellcome Sanger Institute.
3. Clinical Interpretation of Variants in Caner CIViC, identifying evidence levels and clinical trials.
4. Franklin, Variant interpretations.
5. Genome Nexus, OncoKB Therapeutic Levels.
6. Mutalyzer, check HGVS nomenclature, position conversion, SNP converter,...
7. NCBI ClinVar and dBSNP, containing only well documented reportable variants.
8. Varsome, assist in following ACMG and AMP classification.

Please order the test through the desktop little fox.